Fig 1: Generation and characterization of mice with liver-specific single- and double-KOs of Cpt2 and Tsc1.A, Western blot for Cpt2, Tsc1, Hsc70, and mTOR’s downstream substrates in fasted liver extracts from control, Tsc1L-/- (Tsc1), Cpt2L-/- (Cpt2), and Tsc1Cpt2L-/- (DKO) mice (n = 3). B, hematoxylin and eosin staining of livers from control, Tsc1L-/-, Cpt2L-/- and Tsc1Cpt2L-/- mice. The scale bar represents 100 µM. C, liver triglyceride and beta hydroxybutyrate (ßHB) content of fasted control, Tsc1L-/-, Cpt2L-/-, and Tsc1Cpt2L-/- mice (n = 6). D, Western blot for Plin2 and Hsc70 in fasted liver extracts of control, Tsc1L-/-, Cpt2L-/-, and Tsc1Cpt2L-/- mice. One-way ANOVA followed by Tukey’s multiple comparison test was performed where appropriate to detect significance between genotypes. The single letter denotes p < 0.05, and double letters denote p < 0.01. Letters w (for control), t (for Tsc1), c (for Cpt2), and d (for DKO) represent significance between the genotypes. Data are represented as the mean ± SEM. Cpt2L-/-, liver-specific deletion of carnitine palmitoyltransferase 2; DKO, double-KO; TSC1L-/-, liver-specific deletion of TSC1.